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The effects of buffer and pH on GalAK and GalK activities. The activity of GalAK (A) and GalK (B) were analyzed at different buffers (Tris-HCl, phosphate, MES, MOPS, and HEPES) at different pH levels. Except for the buffer, the kinase reactions were performed under standard conditions. Each value is the mean of triplicate reactions, and the values varied by no more than 5%.
