TABLE 3.

The effect of potential inhibitors on GalAK and GalK activities

Inhibitors (at 4 mm), or control (water) were mixed with GalAK or GalK in 100 mm Tris-HCl, pH 7.6, for 10 min on ice prior to performing the enzymatic reaction under standard conditions for each enzyme activity. Each value is the mean of triplicate reactions, and the values varied by no more than ±10%.

Nucleotide inhibitor	Relative GalAK activity	Relative GalK activity
4 mm	%
NAD+	100	111
UTP	105	112
CTP	100	112
GTP	104	107
PPi	109	110
NADP+	93	95
ITP	104	110
ADP	60	77
UDP	105	111
TTP	101	96
AMP	101	95
Control	100	100