FIGURE 2.

Immunoblot analysis of recombinant matrilins with mutated cleavage sites. Matrilin-2 (A), matrilin-3 (B), and matrilin-4 (C) with either wild type hinge region (M2, M3, and M4) or a mutated hinge region, in which the conserved motif EE was replaced by two alanine (M2AA and M4AA) or glutamine residues (M3QQ and M4QQ), were expressed in 293EBNA cells and conditioned media subjected to non-reducing SDS-PAGE on 4–12% gels. After transfer to nitrocellulose, matrilins were detected with antibodies specific for matrilin-2, -3, or -4. For nomenclature, see Footnote 3.