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Processing of matrilins by ADAMTS4 and ADAMTS5 in vitro. Recombinant matrilin-3 and -4 homo-oligomers and matrilin-1/-3 hetero-oligomers were incubated with truncated forms of ADAMTS4 (A) or ADAMTS5 (B) for the indicated periods time at 37 °C. The digests were separated on non-reducing 4–12% SDS-polyacrylamide gels. Bands were visualized with Coomassie Brilliant Blue. For nomenclature see, Footnote 3.
