FIGURE 9.

Overexpression and knockdown of ADAMTS4 and ADAMTS5 in matrilin-4 expressing 293EBNA cells. ADAMTS4 and ADAMTS5 were either overexpressed together with matrilin-4 (A–D) or knocked down by specific siRNAs (E) in matrilin-4 expressing 293EBNA cells. Cell lysates (A and C) or conditioned media (B, D, and E) were subjected to non-reducing SDS-PAGE on 4–12% gels. After transfer to nitrocellulose, matrilins were detected with antibodies specific for the N-terminal neo-epitope of the cleaved C-terminal coiled-coil fragment of matrilin-4 (A and B) (anti-matn4pc) or full-length matrilin-4 (A and B) (anti-matn4). For nomenclature, see Footnote 3.