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Munc18-1 restores membrane transport and localization of Syntaxin1a in Munc18-1 knockdown PC12-KD43 cells. Representative confocal images of PC12-KD43 cells transfected with pCMV5 empty vector (top panel), pCMV5-Munc18-1-WT (middle panel), or pCMV5-Munc18-1-F115E/E132A (bottom panels). Cells were fixed 48 h after transfection and immunostained for Munc18-1 (green) and Sx1a (red). Scale bars, 10 μm.
