TABLE 1.

Thermodynamic parameters for the binding of Sx1a-(2–243) to Munc18-1 determined by ITC

Proteins interaction	ΔH	TΔS	ΔG	Kd	n
		kcal mol−1		nm
Munc18-1-WT-Sx1a-(2–243)	−19.98 ± 0.88	−9.59 ± 1.04	−10.39 ± 0.20	27.27 ± 7.51	1.05 ± 0.01
Munc18-1-F115E/E132A-Sx1a-(2–243)	−14.17 ± 0.17a	−3.79 ± 0.28a	−10.38 ± 0.11	25.77 ± 4.76	0.96 ± 0.01

^a Values were expressed as mean ± S.E. (n = 3, p < 0.01). See ″Experimental Procedures″ for sample concentrations used. All experiments were performed at 298 K.