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. 2009 May 22;284(32):21684–21695. doi: 10.1074/jbc.M109.006254

FIGURE 6.

FIGURE 6.

Complementation of Golgi α-mannosidase II deficiency. Coomassie-stained SDS-polyacrylamide gel run under reducing conditions showing undigested (−) and Endo H-digested (+) s19A expressed alone in HEK 293T (sample 1) and Lec36 cells (sample 2) and of s19A co-expressed in HEK 293T Lec36 cells with wild-type Golgi α-mannosidase II (sample 3), the G574E substituted form of Golgi α-mannosidase II encoded by MA1 (sample 4), and the ΔILVQ-deleted form of Golgi α-mannosidase II encoded by MA2 (sample 5). Open arrowhead, Endo H.