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. 2009 Jun 17;284(32):21765–21775. doi: 10.1074/jbc.M109.004838

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Printor and torsinA interact in vivo. A, co-immunoprecipitation of printor with torsinA WTΔ40. Lysates from HeLa cells expressing either HA-tagged torsinA WTΔ40 or HA vector and Myc-tagged printor were subjected to immunoprecipitation with anti-HA antibody (12CA5). Immunoprecipitates were analyzed by immunoblotting (IB) with anti-HA and anti-Myc antibodies. The asterisks indicate potential degradation products of printor. B, co-immunoprecipitation of printor with full-length torsinA. Lysates from HeLa cells expressing HA-tagged printor or HA vector and C-terminal FLAG-tagged torsinA or FLAG vector were subjected to immunoprecipitation (IP) with anti-FLAG antibody. The immunoprecipitates were analyzed by immunoblotting with anti-FLAG and anti-HA antibodies. C, association of endogenous torsinA and printor in the mouse brain. Homogenates from mouse cerebellum were subjected to immunoprecipitation using anti-printor antibody or the preimmune serum, followed by immunoblot analysis with anti-printor and anti-torsinA antibodies.