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. 2009 Jun 24;284(33):21872–21880. doi: 10.1074/jbc.M109.022749

FIGURE 9.

FIGURE 9.

Effect of MyoD on the interaction of PGC-1α with SIRT1. a, immunoprecipitation analysis of the interaction of PGC-1α with SIRT1. C2C12 cells were transduced with adenoviral vectors for PGC-1α and/or MyoD (left panel), and SIRT1 (right panel), as indicated. Cell extracts were immunoprecipitated (IP) using an antibody directed against PGC-1α and immunoblotted (IB) with an anti-SIRT1 antibody (top panel). Whole cell extracts were probed with the anti-SIRT1 antibody to confirm equivalent SIRT1 input before immunoprecipitation (bottom panels). b, C2C12 cells were transduced with adenoviral vectors for PGC-1α and/or MyoD, and SIRT1, as indicated. Cell extracts were immunoprecipitated using an antibody directed against SIRT1 and immunoblotted with an anti-PGC-1α antibody (top panel). Whole cell extracts were probed with the anti-PGC-1α antibody to confirm equivalent PGC-1α input before immunoprecipitation (bottom panel). c, C2C12 cells were transduced with adenoviral vectors for PGC-1α, MyoD, with or without co-transduction with the SIRT1 vector, as indicated. Cell extracts were immunoprecipitated using an antibody directed against MyoD and immunoblotted with an anti-PGC-1α antibody (top panel). Whole cell extracts were probed with the anti-PGC-1α antibody to confirm equivalent PGC-1α input before immunoprecipitation (bottom panel).