FIGURE 2.

Apoptosis is specific for BMSCs and activated lymphocytes co-culture. PHA-activated and non-activated lymphocytes were added to monolayer of BMSCs. Non-attached lymphocytes were removed after 24 h of co-incubation. The percentage of annexin V-positive attached lymphocytes was detected by fluorescent microscopy (A). Jurkat cells were added to the monolayer of skin fibroblasts in a ratio of 1:10. After the indicated time periods, non-attached Jurkat cells were removed from the co-culture chambers. The percentage of annexin V-positive cells among attached cells was detected by fluorescent microscopy (B). The results of three independent experiments are presented as mean ± S.E.