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Gadd45β expression in primary rat HSC and hepatocytes. Primary rat hepatocytes (parenchymal cells (PC)) were isolated and cultured for 24 h as published recently (12). Primary rat HSC were cultured for 2–14 days as indicated. Then HSC and parenchymal cells were detected for Gadd45β-mRNA expression by reverse transcription-PCR (A) and for Gadd45β-protein expression by Western blot (B), respectively, as described under “Experimental Procedures.” β-Actin served as a loading control.
