FIGURE 1.

Lipin 1 expression is regulated by intracellular sterols in Huh7 cells. Huh7 cells were cultured in medium containing sterols, LPDS, or statin for 24 h. A, LPIN1 mRNA levels were normalized to those of 36B4 mRNA and are expressed relative to the normalized values of cells in cultured sterol-containing medium, set at 1. All values are expressed as means ± S.E. (n = 3). The statistical significance of LPDS or statin treatments versus sterols is indicated as follows: * (p < 0.05) or ** (p < 0.01). B, cell extracts were prepared, and aliquots (50 μg (for lipin 1) or 30 μg (for lamin B2) of protein/lane) were subjected to SDS-PAGE. Immunoblots were probed with anti-lipin-1 antibody or anti-lamin B2 antibody. The experiments were repeated three times.