FIGURE 6.

ChIP assays reveal that SREBP-1 and NF-YA bind to the SRE and NF-Y-binding sites in Huh7 cells, respectively. ChIP assays were performed using LPDS-treated Huh7 cells. Soluble chromatin was immunoprecipitated with preimmune rabbit IgG, anti-SREBP-1, or anti-NF-YA antibody. Immunoprecipitates were subjected to PCR with a primer pair specific to the LPIN1 promoter. As a negative control, a second set of primers was used to amplify another genomic region that was not expected to interact with SREBP-1 or NF-YA. PCR was performed on the total chromatin input. The experiments were repeated three times.