TABLE 1.

Compositions and yields of constructs

Residues are numbered in relation to the initiating methionine for full-length TSP-1. A summary of the recombinant constructs is as follows. There were N-terminal ADPG and C-terminal LELVPRGSAAGHHHHHH tails in E1; N-terminal ADPG and C-terminal GLELVPRGSAAGHHHHHH tails in E2 and E12; N-terminal ADPG and C-terminal ALELVPRGSAAGHHHHHH tails in E3, E23, and E123. Extinction coefficients (EC) are calculated based on the contents of Trp, Tyr, and disulfide bonds. Mass indicates the molecular mass, including the N- and C-terminal tails and neglecting contribution of carbohydrate in E1.