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. 2009 Jun 16;284(33):22206–22212. doi: 10.1074/jbc.M109.026120

TABLE 2.

Denaturation of E12, E23, and E123 as assessed by DSC

Numbers are means of two independent experiments. NC means not calculated.

Protein Tm ΔH
°C kcal/mol
E12 in EDTA 52.2 51
E12 in 0.2 mm Ca2+ 54.9 50
E12 in 2 mm Ca2+ 58.9 50
E23 in EDTA 44.3 NC
E23 in 0.2 mm Ca2+ 49.4 NC
E23 in 2 mm Ca2+ 52.2 NC
E123 in EDTA 59.0 33
E123 in 0.2 mm Ca2+ 62.6 44
E123 in 2 mm Ca2+ 67.6 63