TABLE 2.

Denaturation of E12, E23, and E123 as assessed by DSC

Numbers are means of two independent experiments. NC means not calculated.

Protein	Tm	ΔH
	°C	kcal/mol
E12 in EDTA	52.2	51
E12 in 0.2 mm Ca2+	54.9	50
E12 in 2 mm Ca2+	58.9	50
E23 in EDTA	44.3	NC
E23 in 0.2 mm Ca2+	49.4	NC
E23 in 2 mm Ca2+	52.2	NC
E123 in EDTA	59.0	33
E123 in 0.2 mm Ca2+	62.6	44
E123 in 2 mm Ca2+	67.6	63