FIGURE 6.

Catalase inhibition or the absence of catalase activity is associated with increased oxidative modification of Rpn1 and Rpn2 in the lungs. Lung extracts from acatalasemic mice (A) or mice treated with aminotriazole (B) were incubated with BIAM followed by pull down with streptavidin-agarose. Representative Western blots show the levels of Rpn1 and Rpn2 before (input) and after streptavidin-agarose precipitation (pull down). The -fold changes were calculated as the ratio of the intensity of the precipitated Rpn1 or Rpn2 versus that found in the lung extracts before precipitation. A second experiment provided similar results.