FIGURE 9.

Effects of oxidation of Rpn1 and Rpn2 on binding to the 20 S CP. Control unoxidized or oxidized Rpn1/Rpn2 (i.e. that had been preincubated with GSH (100 μm) and H₂O₂ (200 μm) for 15 min) (A) or oxidized Rpn2 or unoxidized Rpn2 (B) were incubated with purified 20 S CP (0.5 μg) for 10 min. Samples were then resolved in Clear Native PAGE followed by Western blot analysis with subsequent re-probing of the membrane with antibodies specific to Rpn1 or Rpn2, or to the 20 S α-subunit. Representative Western blots are shown. A second experiment provided similar results.