FIGURE 4.

Target identification within the promoter of gp30. A, PAGE-EMSA analysis of the radioactively labeled 342-bp DNA fragment and of its derivatives obtained by digestion with the AluI restriction enzyme. Protein amounts are shown in nanograms. B, scheme presenting a physical map of the 342-bp fragment. The positions of the AluI restriction sites are indicated. The black arrows show the position of the inverted repeat (IR) upstream of the gp30 gene transcription start site. C, sequence comparison of two identified SvtR short targets, 36m (Pgp08) and 39m (Pgp30). Black dots indicate the identical parts present in the inverted repeat region of both sequences. Black arrows show the position of the inverted repeats identified in both promoter regions.