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. 2009 Jun 17;284(33):22222–22237. doi: 10.1074/jbc.M109.029850

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — SvtR represses transcription from two SIRV1 promoters. The transcription regulator activity of SvtR was tested using an IVT. All reactions were performed in the presence of limiting amounts of transcription factors TBP and TFB. The added amounts of SvtR are shown in nanograms. Transcription assays from: gp30 promoter (top panel), gp08 promoter (middle panel), and T6 SSV1 promoter (bottom panel). Strong transcription repression was observed for the Pgp30, a weaker repression for the Pgp08, and no effect was seen with the control T6 promoter from an unrelated virus.