FIGURE 3.
SDS-PAGE and immunoblot analysis of recombinant Kdo transferases from A. aeolicus and E. coli. The proteins were resolved using 10% polyacrylamide gels and stained with Coomassie Blue. Molecular mass protein markers (kDa) were run in lanes M. The asterisks indicate the positions of the supposed WaaA subunits, dimers, trimers, and tetramers. A, SDS-PAGE of purified WaaAAAE (15 μg); B, immunoblot of WaaAAAE (5 μg) probed with anti-histidine tag and alkaline phosphatase-conjugated goat anti-mouse IgG (H + L) antibodies and developed in the presence of nitro blue tetrazolium and 5-bromo-4-chloro-3-indolylphosphate substrate; C, SDS-PAGE of purified WaaAECO (5 μg).