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Sensorgrams of the binding between CMP and the Kdo transferase of A. aeolicus immobilized to a CM5 sensor chip (A) and steady-state analysis of the interaction between CMP and WaaAAAE (B). Req (RU) from two data sets were plotted versus the concentration of CMP (mm). Fitting of the isotherm to a 1:1 Langmuir model resulted in a KD of 401 ± 25 μm.
