TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or Ref. |
|---|---|---|
| E. coli strains | ||
| BW30270 | E. coli K-12 MG1655 rph+fnr+ | CGSC 7925 |
| KPM22 | BW30270 ΔgutQ ΔkdsD yhjD400 | Ref. 28 |
| KPM50 | KPM22 kdsD+ | This study |
| KPM53 | KPM50 ΔwaaC | This study |
| KPM56 | KPM53 ΔwaaA | This study |
| KPM122 | KPM56 with pUM210 carrying the waaA gene of C. psittaci 6BC | This study |
| KPM123 | KPM56 with pUM211 carrying the waaA gene of H. influenzae I69 | This study |
| KPM124 | KPM56 with pUM212 carrying the waaA gene of E. coli K-12 | This study |
| KPM125 | KPM56 with pUM216 carrying the waaA gene of A. aeolicus | This study |
| XL1-Blue | E. coli K-12 recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (TetR)] | Stratagene |
| BL21-CodonPlus(DE3)-RIL | E. coli B F−ompT hsdS(rB– mB–) dcm+ TetRgal λ(DE3) endA Hte [argU ileY leuW CmR] | Stratagene |
| BL21-RIL01 | BL21-CodonPlus(DE3)-RIL with pT7-kdsB | This study |
| BL21-SI | F–ompT lon hsdSB(rB– mB–) gal dcm endA1 proUp ::T7 RNAP ::malQ-lacZ (TetS) | Invitrogen |
| BL21-SI212 | BL21-SI with pUM212 | This study |
| BL21-SI216 | BL21-SI with pUM216 | This study |
| S. enterica sv. Typhimurium strains | ||
| SL3770 | rfa+; smooth form LPS | SGSC 225 |
| SA1627 | Δ(rfb-his); Ra chemotype of LPS | SGSC LPS mutant kit |
| SL3749 | rfaL446; Ra chemotype of LPS | SGSC 228 |
| SL733 | rfaK953; Rb1 chemotype of LPS | SGSC 389 |
| SL3750 | rfaJ417; Rb2 chemotype of LPS | SGSC 229 |
| SL3748 | rfaI432; Rb3 chemotype of LPS | SGSC 227 |
| SL3769 | rfaG471; Rd1 chemotype of LPS | SGSC 231 |
| SL1102 | rfaE543; Re chemotype of LPS | SGSC 258 |
| Corynebacterium glutamicum R163/pJKB72 | Restriction-deficient mutant of C. glutamicum AS019 harboring the expression plasmid pJKB72 with kdsB of E. coli K-12 | Ref. 30 |
| Plasmids | ||
| pT7-7 | AmpR; T7 expression vector | Ref. 79 |
| pKD46 | AmpR; λ Red recombinase expression plasmid | Ref. 27 |
| pKD4 | AmpR, KanR; template plasmid for kanamycin resistance cassette | Ref. 27 |
| pCP20 | AmpR, CmR; FLP recombinase expression plasmid | Ref. 27 |
| pT7-kdsB | pT7-7 carrying the kdsB gene of A. aeolicus | This study |
| pUM140 | Cm+; pHSG398 carrying the waaA gene of C. psittaci 6BC | Ref. 29 |
| pCB23 | KanR; E. coli/C. glutamicum shuttle plasmid carrying the waaA gene of H. influenzae I69 | Ref. 14 |
| pET-16b | AmpR; T7 expression vector for N-terminal His-tag fusions | Novagen |
| pUM210 | pET-16b carrying the waaA gene of C. psittaci 6BC | This study |
| pUM211 | pET-16b carrying the waaA gene of H. influezae I69 | This study |
| pUM212 | pET-16b carrying the waaA gene of E. coli K-12 | This study |
| pUM216 | pET-16b carrying the waaA gene of A. aeolicus | This study |