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. 2009 Jun 16;284(33):22263–22273. doi: 10.1074/jbc.M109.033118

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Association of PARP1 with Sox2 or Oct4. A, CoIP of FLAG-PARP1 and HA-Sox2 in HEK 293 cells, which were transiently cotransfected with FLAG-PARP1 and HA-Sox2 or vector. B, association of endogenous PARP1 with Sox2 or Oct4 in F9 and P19 cells. The NE of F9 or P19 cells was subjected to CoIP with anti-Sox2 antibody or anti-Oct4 antibody. Anti-GST antibody was used as a negative control. C, association of endogenous PARP1 with Sox2 in PARP1^+/+ and PARP1⁻^/⁻ ESCs. D, Sox2 but not Oct4 interacts directly with PARP1 in vitro. A GST-pulldown assay was performed with purified GST-Sox2, GST-Oct4, and His-PARP1 fusion proteins. The representative results of three independent experiments are shown. IP, immunoprecipitation; WB, Western blot.