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. 2009 May 19;284(33):22364–22378. doi: 10.1074/jbc.M901528200

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — A, anti-rwBmPAL antibodies bind to a Wolbachia product present within BMFE. Anti-rwBmPAL polyclonal antibody immunoblot of three batches of BMFE (a–c) and a corresponding preparation of Wolbachia-depleted BMFEtet, separated by one-dimensional SDS-PAGE. Molecular weight markers are stated in kilodaltons. B, anti-rwBmPAL staining of Wolbachia-infected C6/36 cells (an A. albopictus mosquito cell line, top right panel) and Wolbachia-free C6/36 cells (top left panel). Antibody reactivity was detected with goat anti-rabbit FITC conjugate and counterstained with Evans Blue. Human onchocercoma sections (lower left panel) and B. malayi adult females (lower right panel) were stained using the affinity-purified anti-rwBmPAL antibody. Antibody reactivity was detected using alkaline phosphatase with a permanent red chromogenic substrate and counterstained with hematoxylin.