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. 2009 May 19;284(33):22364–22378. doi: 10.1074/jbc.M901528200

FIGURE 3.

FIGURE 3.

Synthetic diacyl-lipopeptide analogue of wBmPAL (Diacyl WoLP) replicates BMFE-TLR2/6-specific activation of inflammation. A, HEK-TLR2 cells were transfected with plasmids encoding small interfering (psi) RNA specific for TLR1 or TLR6 before being stimulated with Diacyl WoLP or Triacyl WoLP (doses stated are in micrograms/ml). Accumulations of IL-8 secreted by HEK-psiTLR1 or -psiTLR6 triplicate cultures 20 h post-stimulation are plotted as mean ± 1 S.E. percentages of corresponding HEK-TLR2 responses (mean ± 1S.E. max IL-8 concentrations are as follows: PAM3CSK = 9807 ± 175 pg/ml, FSL-1 = 2001 ± 345 pg/ml, Diacyl WoLP = 8195 ± 199 pg/ml, Triacyl WoLP 571 ± 27 pg/ml, and BMFE = 9495 ± 137 pg/ml). Significant differences compared with HEK-TLR2 responses are indicated: ***, p < 0.001; **, p < 0.01; and *, p < 0.05. B, peritoneal macrophages from WT, TLR1−/−, and TLR6−/− were stimulated with Diacyl WoLP or Triacyl WoLP and control TLR1/6 ligands PAM3CSK4 and FSL-1 (doses stated are in nanograms/ml) in triplicate, and production of TNFα after 20 h is plotted as mean ± 1S.E. All data are representative of three independent experiments.