FIGURE 5.

siRNA-mediated silencing of endogenous myosin Vb, Rab11a, or Rab11-FIP2 attenuates the transport of NPC1L1 to PM. A, real-time PCR showing the knockdown efficiency of myosin Vb, Rab11a, or Rab11-FIP2. B, CRL1601/NPC1L1-EGFP cells were transfected with the indicated siRNAs. 72 h after transfection, the cells were depleted of cholesterol for different durations of time. At each indicated time point, the cells were fixed and examined by confocal microscopy. Images show the cells representative of the whole cell population at each indicated time point. Bar, 10 μm. C, quantification of the intracellular localization of NPC1L1-EGFP shown in B. Error bars represent standard deviations. Results shown are representative of three independent experiments.