FIGURE 7.

Myosin Vb-CT, Rab11a(S25N), or Rab11-FIP2-(446–511) impairs the NPC1L1-mediated cholesterol uptake. A, NPC1L1-RFP was co-expressed with EGFP-tagged myosin Vb-CT, Rab11a(S25N), and Rab11-FIP2-(446–511) individually in CRL-1601 cells. 48 h after transfection, the cells were depleted of cholesterol followed by cholesterol replenishment for 60 min. Then the cells were fixed, stained with filipin, and examined by two-photon confocal microscopy. Images show the cells representative of the whole cell population at each indicated time point. Bar, 10 μm. Arrows indicate the cells with no expression of the transfected genes. Arrowheads indicate the cells expressing the transfected genes. B, quantification of the intracellular cholesterol of the cells shown in A. Error bars represent standard deviations. Results shown are representative of two independent experiments.