Table 1.

Oligonucleotide primer sequences for renin-angiotensin components and NOS isoforms

	Forward Primer	Reverse Primer	Base Pairs	References
Angiotensin type 1 receptor	5′-GCATCATCTTTGTGGTGGG-3′	5′-ATCAGCACATCCAGGAATG-3′	689	55
Angiotensin-converting enzyme	5′-GCAGTACAAAGACTTGCCTG-3′	5′-TGGCAGAGGCTGACATGTTA-3′	599	45
Angiotensinogen	5′-TTGTTGAGAGCTTGGGTCCCTTCA-3′	5′-CAGACACTGAGGTGCTGTTGTCCA-3′	280	25
GAPDH	5′-ACCACAGTCCATGCCATCAC-3′	5′-TCCACCACCCTGTTGCTGTA-3′	425	6
Endothelial NOS	5′-CCGGGACTTCATCAATCAGT-3′	5′-TGTTGTATCGGTGAGGGTCA-3′	724	http://frodo.wi.mit.edu/
Inducible NOS	5′-CCAACAACACAGGATGACC-3′	5′-CCTGATGTTGCCACTGTTAG-3′	603	39
Neuronal NOS	5′-TGGAGGAGAGGAACACTGCT-3′	5′TCTCTGAAGACGCCCTTGTT-3′	743	http://frodo.wi.mit.edu/

NOS, nitric oxide synthase. Sequences were obtained either from the indicated literature citations or generated using Primer 3 software (version 0.4.0) available at http://frodo.wi.mit.edu/.