Figure 7. Microarray analysis of splenic cDCs from WT, IFN-β-/- and IFNAR-/- mice showed down-regulation of heat shock protein 70.1 and 70.3 (Hsp70.1 and Hsp70.3) in knockout mice.
(A) Affymetrix gene array analysis of splenic cDCs. RNA was prepared from sorted splenic CD8α+DCs and myeloid DCs (CD8α-) from WT, IFN-β-/- and IFNAR-/- mice. All samples were repeated twice with individually sorted cells and averaged. (B) Quantitative real-time PCR (qRT-PCR) of RNA from splenic cDCs sorted from WT, IFN-β-/- and IFNAR-/- mice. (C) qRT-PCR of RNA from splenic cDCs untreated and in vitro treated with 0.1 U/ml and 5 U/ml of murine recombinant IFN-β for 3h. (D) qRT-PCR of RNA from splenic cDCs 24h after administration of poly I:C. Results are representative of at least three samples from WT, IFN-β-/- and IFNAR-/- mice in three independent experiments.