Figure 4. The Iron Regulatory Hormone Hepcidin Supports HCV Replication.
(A) HAMP silencing blocks replication of the full-length HCV replicon OR6. OR6 cells were transfected with the indicated siRNAs for 72 hr and then assayed for luciferase activity (black bars), HAMP transcript levels (white bars), and cellular ATP content (gray bars). Values were obtained from quadruplicate wells in three independent experiments and are mean ± SD.
(B) HAMP silencing blocks replication of the infectious JFH1 HCV strain in Huh7 cells. Two-tailed p value was calculated by indepdendent two-sample t test. Values were obtained from triplicate qPCR replicates from duplicate wells in two independent experiments and are mean ± SD.