FIGURE 7.

SARS-CoV nsp3 is associated with IRF-3 in cells infected with SARS-CoV. MA104 cells were mock-infected (lane 1), infected with SeV for 10 h (lane 2), infected with SARS-CoV (m.o.i. = 10) for 18 and 28 h (lanes 3 and 4), respectively, or infected with SARS-CoV for 18 h and then superinfected with SeV for an additional 10 h (lane 5). Expression of IRF-3, RIG-I, MxA, ISG56, SARS-CoV NC, and nsp3, and SeV proteins in cell lysates was determined by immunoblot (IB) analysis (upper panels). A nonspecific band detected by the rabbit anti-MxA antiserum (bottom panel) indicates equal loading. Data shown are representative of three independently conducted experiments. In lower panels, the cell lysates were subjected to immunoprecipitation (IP) with a rabbit anti-IRF3 antiserum, followed by immunoblot analysis of SARS-CoV nsp3, and IRF-3 (using an mAb anti-IRF3 antibody). Data shown are representative of two independently conducted experiments.