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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Leuk Res. 2009 Feb 3;33(11):1525–1529. doi: 10.1016/j.leukres.2009.01.002

Figure 4. Comet assay to study the effect of JS-K and ARA-C on HL-60 cell DNA.

Figure 4

A- HL-60 cells were treated with the indicated concentrations of each agent for 24 hours whereupon the comet assay for DNA strand breaks was conducted as described in the text. At a concentration of 0.5 μM, JS-K by itself did not induce significant DNA damage. ARA-C induced DNA damage at submicromolar concentrations. B- JS-K and ARA-C were added simultaneously to HL-60 cells at the indicated concentrations of each drug. After 24 hours, the comet assay was conducted as detailed in the text. JS-K significantly augmented DNA damage induced by ARA-C (images are representative of 3 independent experiments). C – DNA single-strand breaks were quantified on the basis of fluorescence intensity of the single-cell comet tails after gel electrophoresis. Results are expressed as average ± SE of 50 comets per sample and are representative of 3 separate experiments. Cells treated with ARA-C alone had significantly (P<0.001) greater level of DNA damage compared with untreated controls. Lower concentrations of JS-K and ARA-C combined resulted in significantly (P<0.001) more DNA strand break damage than higher doses of ARA-C alone.