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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: J Immunol. 2008 Dec 15;181(12):8633–8641. doi: 10.4049/jimmunol.181.12.8633

Figure 2. AMPK activity is rapidly modulated by anti-inflammatory and proinflammatory stimuli.

Figure 2

A and B, Anti-inflammatory cytokines IL-10 and TGFβ enhance the levels of phosphorylated AMPK in mouse macrophages. Bone marrow derived macrophages were stimulated with 20 ng/ml IL-10 or 5 ng/ml TGFβ for the time points indicated. C, LPS stimulation diminishes AMPK phosphorylation in mouse macrophages. Bone marrow derived macrophages were stimulated with 100 ng/ml LPS for the time points indicated. D, LPS stimulation decreases AMPK phosphorylation in human macrophages. Human macrophages were stimulated with 100 ng/ml LPS for the time points indicated. Western blot was performed using antibodies against phospho-AMPKα and total AMPKα. The p-AMPKα/AMPKα ratio for each was analyzed by densitometry and shown as bar graphs. Data shown are representative of 4 independent experiments with similar results.