. Author manuscript; available in PMC: 2010 Jun 2.

Published in final edited form as: Biochemistry. 2009 Jun 2;48(21):4506–4518. doi: 10.1021/bi802206k

Table 4.

Thermodynamic parameters of bupropion binding to the Torpedo AChR in different conformational states determined by non-linear chromatography studies and van’t Hoff analysis.

Thermodynamic parameters	Condition
Thermodynamic parameters	α-BTx treated column¹	Epibatidine treated column²
ΔH° (kJ mol⁻¹)	−5.0 ± 0.2	−5.7 ± 1.3
ΔS° (J mol⁻¹ K⁻¹)	89 ± 1	85 ± 5
ΔG²⁰ (kJ mol⁻¹)	−31.0 ± 0.4	−30.6 ± 1.4

The CMAC-Torpedo AChR was pretreated with either α-BTx¹ (the AChR is mainly in the resting state) or epibatidine² (the AChR is mainly in the desensitized state)

The thermodynamic parameters ΔH° and ΔS° were calculated from Fig. 5 according to eq. 8, and the ΔG²⁰ values were calculated using eq. 9.