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. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: Biochemistry. 2009 Apr 21;48(15):3417–3424. doi: 10.1021/bi8023288

Fig 2.

Fig 2

MnSOD activity and the effect of the Y34N mutation. (A) The increase in absorbance (absorbance units) at 420 nm of 120 mM Y34N MnSOD in the 2 ms after pulse radiolysis generated initial superoxide concentrations of 5 mM. (B) The same experiment at longer times. Solutions contained 30 mM formate, 50 µM EDTA, and 2 mM Taps at pH 8.2. The enzyme had been reduced prior to the experiment with 130 mM H2O2. The data are fit to eq 1–eq 4 resulting in the entries to Table 2.