Skip to main content
. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: Biochemistry. 2009 Apr 21;48(15):3417–3424. doi: 10.1021/bi8023288

Fig 5.

Fig 5

Molar extinction coefficient (M−1cm−1) observed after pulse radiolysis of a solution of Y34N MnSOD (182 µM) at pH 7.1 pre-reduced with H2O2. (Open squares) spectrum of Mn3+(OH)SOD obtained by extrapolating to long times in Figure 2A; (filled diamonds) spectrum of the product-inhibited complex obtained by extrapolating the initial increase (t # 0.2 ms) as in Figure 2A; (open diamonds) spectrum of a newly-detected intermediate obtained by extrapolating the second kinetic process at times near 2 ms as in Figure 2B. Solutions contained 30 mM formate, 50 µM EDTA, and 2 mM Mops.