Table 1.

Parameters for Na⁺ current activation and inactivation

		Activation Vh-70 mV			Activation Vh-140 mV			Steady-state inactivation (500 ms prepulse)			Fast inactivation (15 ms prepulse)
		V1/2	k	n	V1/2	k	n	V1/2	k	n	V1/2	k	n
Nav1.8	control	−5.7±0.4	5.1±0.2	8	−5.2±1.0	5.2±0.2	8	−49.0±1.2	9.6±0.3	8	−29.5±1.8	10.9±0.8	8
	isoflurane	−5.7±0.7	5.6±0.1	8	−6.6±1.3	5.4±0.3	8	−54.6±1.3**	10.8±0.4**	8	−41.7±2.4**	13.1±1.0	8
TTX-s	control	−20.9±1.2	5.3±0.3	6	−24.5±0.9	5.5±0.2	6	−67.0±2.3	6.0±0.3	4	−55.7±0.6	13.6±0.4	4
	isoflurane	−21.6±0.9	5.8±0.3	6	−25.9±1.3	5.7±0.4	6	−74.1±2.1***	6.5±0.4	4	−70.7±2.6**	16.6±20.	4

Mean values were derived from Boltzmann equation fits of individual data sets as described in the Methods. Isoflurane was tested at 0.53±0.06 mM (equivalent to ~1.8 MAC when corrected to 24°C).

Abbreviations: k, slope; n, number of experiments; V_h, holding potential; V_1/2, voltage at which half maximal activation or inactivation occurs.

^**p < 0.01;

^***p<0.001 vs. control, compared by paired two-tailed student t-test.