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. Author manuscript; available in PMC: 2009 Oct 2.
Published in final edited form as: J Neurophysiol. 2006 Aug 16;96(5):2488–2500. doi: 10.1152/jn.00593.2005

FIG. 4.

FIG. 4

Pharmacological characterization of spontaneous EPSCs in hippocampal neuronal cultures. A: typical record of spontaneous, ongoing synaptic activity (EPSCs) in a voltage-clamped cultured hippocampal neuron (left). Activity recorded at time points 1 and 2 are magnified (right) to illustrate small (A1) and compound (A2) EPSCs. B: nearly all synaptic activity was abolished by the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor antagonist CNQX (10 μM, n = 12). C: NMDAR antagonist MK-801 (10 μM, n = 10) had little effect on EPSC frequency. D: voltage-sensitive Na+ channel blocker tetrodotoxin (TTX, 1 μM, n = 5) did not affect spontaneous small EPSCs, but eliminated compound EPSCs, suggesting that these large events were the result of presynaptic action potentials.