Figure 3.

Effect of chelating agents on [Ca²⁺]_i elevations in platelets interacting with acid-soluble type I collagen under flow. Blood cell suspensions (Figure 1 legend contains details) were perfused over collagen at the shear rate of 250 seconds without or with prior treatment with a Ca²⁺ chelator, the membrane impermeable EGTA (5 mM), the membrane permeable BAPTA-AM (80 μM), or the 2 combined. Results are expressed as described in the legend to Figure 2 (n = 3).