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. 2009 Aug 19;29(33):10221–10233. doi: 10.1523/JNEUROSCI.1404-09.2009

Figure 7.

Figure 7.

Effect of train stimulation on FM1-43 destaining of synaptic vesicle clusters. Aa, FM1-43-labeled presynaptic terminals, before (top) and after (bottom) destaining at 1 Hz in control. Ab, The black trace shows control destaining versus stimulus number of the terminals arrowed. The same presynaptic axon and terminals were restained with FM1-43 (Ac), and then superfused with 5-HT (600 nm). Stimulation (1 Hz) subsequently failed to destain (Ab; blue). Ad, Mean of data plotted as for Ab from all five preparations. Ba, Presynaptic terminals (examples arrowed) labeled as in A but destained with action potentials in trains (10 at 50 Hz; 10 s intervals) with the same total number of stimuli as in A. In control, destaining was indistinguishable from 1 Hz destaining (Bb, black). Axons and presynaptic terminals were restained (Bc) and reimaged. In 5-HT (600 nm), partial destaining was observed (blue). Bd, Mean of data plotted from all four preparations. Error bars indicate SEM.