Table 1.
Nitrocefin |
Ampicillin |
|||||
---|---|---|---|---|---|---|
Protein | kcat (s−1) | Km (μM) | kcat/Km (μM−1 s−1) | kcat (s−1) | Km (μM) | kcat/Km (μM−1 s−1) |
wild-type | 110 ± 10 | 1.9 ± 0.3 | 61 ± 11 | 500 ± 48 | 13 ± 4 | 40 ± 12 |
S67G | 0.052 ± 0.003 | 61 ± 8 | 0.00086 ± 0.00010 | < 0.002 | ND | ND |
K70A | 0.024 ± 0.003 | 0.72 ± 0.33 | 0.033 ± 0.016 | < 0.002 | ND | ND |
K70E | 0.020 ± 0.001 | 1.2 ± 0.3 | 0.017 ± 0.004 | < 0.002 | ND | ND |
K70D | < 0.002 | ND | ND | < 0.002 | ND | ND |
ND – not determined