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. Author manuscript; available in PMC: 2010 Jul 7.

Published in final edited form as: Biochemistry. 2009 Jul 7;48(26):6136–6145. doi: 10.1021/bi900448u

Table 1.

Kinetic constants for OXA-1 wild-type and mutants

	Nitrocefin			Ampicillin
Protein	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹ s⁻¹)	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹ s⁻¹)
wild-type	110 ± 10	1.9 ± 0.3	61 ± 11	500 ± 48	13 ± 4	40 ± 12
S67G	0.052 ± 0.003	61 ± 8	0.00086 ± 0.00010	< 0.002	ND	ND
K70A	0.024 ± 0.003	0.72 ± 0.33	0.033 ± 0.016	< 0.002	ND	ND
K70E	0.020 ± 0.001	1.2 ± 0.3	0.017 ± 0.004	< 0.002	ND	ND
K70D	< 0.002	ND	ND	< 0.002	ND	ND

ND – not determined