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. Author manuscript; available in PMC: 2010 Jul 7.
Published in final edited form as: Biochemistry. 2009 Jul 7;48(26):6136–6145. doi: 10.1021/bi900448u

Table 2.

Deacylation rates of OXA-1 K70 mutants

Substrate Buffer Deacylation rate k3 (s−1)
K70A K70E K70D
Bocillina 50 mM NaH2PO4, pH 7.0 0.00022±0.00002 < 4.0 × 10−5 < 4.0 × 10−5
Ampicillinb 50 mM NaH2PO4, pH 7.0 0.00012±0.00001 < 4.0 × 10−5 < 4.0 × 10−5
50 mM NaH2PO4, 9 mM Na2CO3, pH 7.0 0.00090±0.00004 < 4.0 × 10−5 < 4.0 × 10−5
Ampicillinb 50 mM NaH2PO4, pH 7.4 0.00023±0.00001 ND ND
100 mM NaH2PO4, pH 7.4 0.00034±0.00001 ND ND
50 mM Tris-Cl, pH 7.4 < 4.0 × 10−5 ND ND
50 mM NaH2PO4, 9 mM Na acetate, pH 7.4 0.0056 ± 0.0004 < 4.0 × 10−5 < 4.0 × 10−5
50 mM NaH2PO4, 9 mM Na propanoate, pH 7.4 0.0096 ± 0.0006 < 4.0 × 10−5 < 4.0 × 10−5
a

Measured by SDS-PAGE assay.

b

Measured by competition with Cibacron Blue 3GA. ND – not determined