Figure 1. Peroxide induces AMPK activation in endothelium.

PAECs in 6-well plates were exposed to H₂O₂ as indicated, lysed, and the lysates probed for (A) phosphorylation of AMPK and ACC, (B) AMPK activity, and (C) ATP content as described in “Methods.” PAECs were then treated with H₂O₂ as indicated after treatment with either the AMPK inhibitor, compound C (25 uM; D) or dominant-negative AMPK adenovirus (E) and cell death or viability determined by LDH release and MTS assay, respectively as described in “Methods.” (F) PAECs were treated with dominant-negative AMPK adenovirus and AMPK activation assessed after H₂O₂ exposure by phosphorylation of the AMPK target, acetyl-CoA carboxylase (ACC).