TABLE 1.

Strain and plasmid list

The abbreviations used are as follows: Amp, ampicillin; Kan, kanamycin.

Strain or plasmid	Genotype	Resistance	Source or Ref.
Escherichia coli
DH5α-E	Cloning strain	None	Invitrogen
BW25141	Cloning strain	None	15
ER2566	Protein expression strain	None	New England Biolabs
Staphylococcus aureus
RN4220	Restriction modification-deficient	None	32
ROJ143	Δagr ::Erm pAgr-lux/pAgrC1agrA	Cam	24
SH1000	sigB+ derivative of NCTC8325-4	None	33
SH1001	SH1000/Δagr ::tetM	Tet	33
AH430	SA502A/pDB59	Cam	23
Plasmids
pAgrD1	pEPSA5/agrBD	Amp, Cam	This work
pAgrD7a	pEPSA5/agrB	Amp, Cam	This work
pAIP1	pBAD18/agrBD	Amp	This work
pBAD18	Arabinose-inducible expression vector	Amp	17
pCOLADuet-1b	Expression vector	Kan	Novagen
pEPSA5	Expression vector	Amp, Cam	18

^a The AgrD C-terminal truncation and point mutants built on pEPSA5 are not listed.

^b The AgrD C-terminal truncation and point mutants built on pCOLADuet-1 are not listed.