Figure 7.
Inhibition of pericellular proteolysis of OCI-AML3 cells. (A) Effects of peptides (200 μmol/L) on the release of gelatin fragments from coated FITC-labeled gelatin. The results show means ± SD from triplicates. *P < .005. (B) Biotinylated cell surface proteins were isolated from cells incubated without peptide (lane 2), with DFEDHD (lane 3), or with HFDDDE (lane 4). Samples were run on sodium dodecyl sulfate–polyacrylamide gel electrophoresis followed by silver staining. Molecular weight markers are shown in lane 1. (C) Quantitation of cell-surface biotinylated proteins with streptavidin-phycoerythrinin flow cytometry (top panel). Immunoblotting with β2 integrin antibody (bottom panel).