Figure 4.

Regulation of GPVI-mediated SHIP-1 phosphorylation by Fyn, Src, and Lyn in murine platelets. Washed and indomethacin-treated WT, Fyn^−/− (A-B), Src^−/− (C-D), and Lyn^−/− (E-F) murine platelets were stimulated with 60 ng/mL CVX or 20 μg/mL collagen under stirring conditions for 60 seconds at 37°C. The reaction was stopped using 3× sample loading buffer (with DTT). The lysates were subjected to Western blotting analysis and probed for pSHIP-1 (Y1020). Total Erk and β-actin served as lane-loading controls.