Figure 1.
EAU and related immune responses in WT and GKO mice. GKO and control WT mice were immunized with 150 μg IRBP emulsified in CFA and received 0.5 μg of PT IP. (A) The EAU score was evaluated by histopathology at 21 days after immunization. The EAU score is the average of all the mice in the group. EAU incidence (positive/total) is shown in each bar. Shown is a representative experiment of three. (B) DTH responses were elicited on day 19 and were evaluated on day 21 after immunization. (C) Ag-specific proliferation of lymph nodes from immunized mice was determined by incorporation of [3H]thymidine. Shown are counts per minute from one representative experiment of three (average of triplicates). *Statistically significant difference in scores versus WT (P < 0.05).