Table 2.
Gene Expression in EAU Onset Eyes, Draining Lymph Nodes, and IRBP-Stimulated T cells
| Eyes* |
Lymph Nodes† |
Cultured T Cells‡ |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Gene | WT | GKO | WT:GKO§ | WT | GKO | WT:GKO§ | WT | GKO | WT:GKO§ |
| Ccl1 | 2.17 | 3.38 | −1.56 | 1.05 | 1.90 | −1.80 | 1.54 | 2.57 | −1.67 |
| Ccl11 | 0.95 | 1.57 | −1.65 | 1.17 | 2.05 | −1.75 | 1.90 | 1.98 | |
| Ccl12 | 1.48 | 1.63 | 0.93 | 0.98 | 1.17 | 0.99 | |||
| Ccl17 | 2.43 | 3.99 | −1.64 | 1.30 | 1.86 | 1.45 | 7.99 | −5.51 | |
| Ccl19 | 1.09 | 1.38 | 1.10 | 1.48 | 1.21 | 0.98 | |||
| Ccl2 | 1.91 | 1.77 | 1.61 | 1.02 | 1.58 | 1.24 | 1.01 | ||
| Ccl20 | 0.87 | 1.07 | 1.37 | 1.33 | 0.93 | 0.98 | |||
| Ccl21 | 1.67 | 2.41 | 1.09 | 2.45 | −2.25 | 0.95 | 1.96 | −2.06 | |
| Ccl22 | 1.68 | 2.72 | −1.62 | 0.96 | 1.70 | −1.77 | 1.59 | 2.93 | −1.84 |
| Ccl24 | 1.60 | 1.92 | 1.12 | 2.81 | −2.51 | 2.17 | 0.97 | 2.24 | |
| Ccl25 | 0.84 | 1.27 | 0.89 | 1.13 | 1.34 | 0.98 | |||
| Ccl27 | 1.22 | 0.99 | 1.01 | 0.79 | 1.26 | 0.98 | |||
| Ccl28 | 1.33 | 1.70 | 0.96 | 1.37 | 1.22 | 1.00 | |||
| Ccl3 | 1.20 | 2.12 | −1.76 | 1.45 | 1.42 | 1.24 | 1.52 | ||
| Ccl4 | 0.95 | 0.96 | 1.46 | 1.18 | 0.91 | 1.00 | |||
| Ccl5 | 3.61 | 1.16 | 3.11 | 2.92 | 1.21 | 2.41 | 3.03 | 0.93 | 3.26 |
| Ccl6 | 1.35 | 1.10 | 1.03 | 1.22 | 1.32 | 0.97 | |||
| Ccl7 | 1.29 | 1.54 | 0.74 | 1.37 | 1.11 | 0.98 | |||
| Ccl8 | 1.30 | 1.10 | 0.93 | 1.24 | 1.61 | 0.97 | 1.66 | ||
| Ccl9 | 1.14 | 1.02 | 1.23 | 0.77 | 1.66 | 0.99 | 1.68 | ||
| Cx3cl1 | 1.28 | 1.16 | 1.32 | 0.91 | 1.30 | 1.01 | |||
| Cxcl1 | 0.96 | 1.13 | 1.00 | 0.88 | 1.10 | 0.96 | |||
| Cxcl10 | 4.12 | 2.60 | 1.58 | 3.82 | 1.71 | 2.23 | 6.04 | 1.96 | 3.08 |
| Cxcl11 | 2.62 | 1.49 | 1.76 | 1.00 | 1.03 | 5.53 | 0.98 | 5.64 | |
| Cxcl12 | 0.90 | 1.23 | 1.03 | 0.91 | 1.51 | 1.27 | |||
| Cxcl13 | 1.14 | 0.87 | 1.72 | 1.58 | 1.42 | 0.98 | |||
| Cxcl14 | 1.31 | 0.96 | 1.50 | 1.37 | 1.10 | 0.98 | |||
| Cxcl15 | 1.21 | 1.75 | 0.82 | 1.43 | 1.51 | 1.00 | 1.51 | ||
| Cxcl16 | 1.10 | 1.03 | 1.53 | 1.79 | 1.07 | 1.00 | |||
| Cxcl2 | 1.31 | 1.74 | 1.17 | 1.21 | 1.38 | 2.97 | −2.15 | ||
| Cxcl4 | 1.45 | 1.14 | 1.58 | 0.96 | 1.65 | 1.24 | 0.99 | ||
| Cxcl5 | 0.87 | 1.13 | 1.45 | 1.35 | 1.19 | 1.02 | |||
| Cxcl7 | 1.30 | 1.18 | 0.93 | 1.34 | 1.46 | 1.00 | |||
| Cxcl9 | 3.75 | 1.57 | 2.39 | 2.75 | 1.17 | 2.35 | 4.95 | 1.99 | 2.49 |
| Xcl1 | 1.28 | 1.13 | 0.99 | 0.95 | 1.54 | 1.05 | |||
*
m RNA levels are expressed as ratio of inflamed eyes to normal eyes (i.e. increase over baseline).
†
mRNA levels in naïve lymph nodes are baseline for draining lymph nodes 3 days after immunization.
‡
The baseline for IRBP-stimulated T cells is the mRNA level in the cells cultured with medium alone.
§
Difference in expression between WT and GKO is shown as the ratio of increase in WT mice to the increase in GKO mice. Each value represents the relative increase in expression for a given chemokine in WT versus GKO mice. Minus values indicate that the expression in GKO mice is higher than in WT mice.